KINETICS AND INHIBITION STUDY OF ACID PHOSPHATASES FROM CAMEL LIVER

Mehrin Sherazi; Asma Saeed; Shakil Abbas; Umber Zaman; Rubina Naz

Mehrin Sherazi Department of Chemistry, Gomal University, Dera Ismail Khan, Pakistan.
Asma Saeed Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan.
Shakil Abbas Department of Chemistry, Gomal University, Dera Ismail Khan, Pakistan.
Umber Zaman Department of Chemistry, Gomal University, Dera Ismail Khan, Pakistan.
Rubina Naz Department of Chemistry, Gomal University, Dera Ismail Khan, Pakistan.

Keywords: Acid phosphatase, Camel liver, Kinetics and inhibition

Abstract

The camel liver contained two acid phosphatases and these were separated by Sephadex G-100 column chromatography. The first activity peak was designated as high molecular weight acid phosphatase (HM-AP)while the second peak was referred as low molecular weight acid phosphatase (LM-AP). Both enzymes were inactivated by 10 mM Cu⁺⁺ and Hg⁺⁺. HM-AP was inhibited by fluoride and tartrate and unaffected by iodoacetate but LM-AP showed opposite behaviour. Phosphate, vanadate, molybdate, tartrate and fluoride were found competitive inhibitors. Their K_i values for HM-AP were 0.6 mM, 0.8µM, 0.18µM, 0.1mM and 0.06mM , respectively while K_i values for LM-AP were1.8mM, 183µM and 317µM, respectively and no inhibitions were observed with tartrate and fluoride.